Peptide Reconstitution & Dosage Calculator

A free peptide calculator for Australian researchers. Convert vial size, bacteriostatic (BAC) water volume, and target dose into the exact number of insulin-syringe units to draw — with presets for popular research peptides. For in-vitro laboratory research only.

Inputs

mg
mL

Results

Draw to this mark

5units

on a 1.0 mL insulin syringe

Concentration

5

mg / mL

Doses per vial

40

250 mcg per aliquot

For in-vitro laboratory research only. Not for human consumption. Always verify the calculation independently before any research use.

How to use the calculator

  1. Enter the vial size in milligrams (printed on the vial label).
  2. Enter the BAC water volume you added during reconstitution.
  3. Enter the target dose per aliquot and pick the unit (mcg or mg).
  4. Select your insulin syringe size — 1.0 mL / 100 unit is the standard choice.
  5. The calculator outputs the syringe units to draw, plus concentration and doses-per-vial so you can cross-check.

New to reconstitution? See our step-by-step reconstitution guide and storage guide.

Worked example

Say you have a 10 mg peptide vial, you reconstitute it with 2 mL of bacteriostatic water, and your target research dose is 250 mcg per aliquot, using a 1 mL / 100-unit insulin syringe:

  • Concentration = 10 mg ÷ 2 mL = 5 mg/mL (5,000 mcg/mL)
  • Volume per dose = 250 mcg ÷ 5,000 mcg/mL = 0.05 mL
  • Syringe units = 0.05 mL × 100 units/mL = 5 units
  • Doses per vial = 10,000 mcg ÷ 250 mcg = 40 doses

So you would draw 5 units on the syringe per aliquot. Enter the same four values above and the calculator returns this instantly — change the BAC water volume or syringe size and it re-runs.

Frequently asked questions

It converts the three values every researcher needs — vial size (mg), bacteriostatic water added (mL), and target dose per aliquot (mcg or mg) — into the exact number of syringe units to draw. This removes the manual arithmetic error that's the most common source of measurement mistakes in peptide research.

Concentration is vial mass divided by BAC water volume. Volume per dose is target dose divided by concentration. Syringe units are that volume scaled to the syringe markings — a 1 mL insulin syringe is graduated into 100 units, so 0.1 mL = 10 units. The calculator does all three steps and shows the intermediate concentration so you can verify it.

1 mL (100 unit) insulin syringes are the most common choice in peptide research because the 100-unit scale gives single-unit precision for typical sub-millilitre doses. 0.5 mL (50 unit) and 0.3 mL (30 unit) syringes give finer resolution for very small doses. Choose the smallest syringe that comfortably fits your dose volume without overdrawing.

Most researchers reconstitute a 10 mg peptide vial with 1–2 mL of bacteriostatic water. More water means a lower concentration and a larger volume to draw per aliquot, which improves measurement accuracy but increases the volume drawn. Stronger concentrations (less water) are useful when target amounts are large.

No — the math is purely volumetric. The presets pre-fill commonly used research starting values for popular peptides — including Retatrutide, BPC-157, GHK-Cu, Semax, and Melanotan II — but they are not protocol recommendations. Your reconstitution volume, dose, and frequency should follow your own research protocol or the published literature for the compound.

Reconstituted peptide solutions are typically stable for 28 days when stored at 2–8°C (refrigerated) and prepared with bacteriostatic water (which contains 0.9% benzyl alcohol as a preservative). Lyophilised vials remain stable for up to 24 months at -20°C. See our Peptide Storage Guide for full guidance.

Doses per vial equals the vial size divided by the dose per aliquot. For example, a 10 mg vial drawn at 250 mcg per aliquot yields 40 doses (10 mg = 10,000 mcg; 10,000 ÷ 250 = 40). The calculator shows doses-per-vial automatically alongside the syringe units so you can plan how long a vial will last and cross-check the maths.

1 mg (milligram) equals 1,000 mcg (micrograms). Peptide vials are usually labelled in mg (e.g. a 10 mg vial), while research doses are often expressed in mcg (e.g. 250 mcg). Mixing the two up is the most common reconstitution error — the calculator lets you select mcg or mg for the target dose so the conversion is handled for you.

The arithmetic itself is simple, but a single decimal-place or mcg/mg slip changes the drawn volume by an order of magnitude. A calculator removes that error, shows the intermediate concentration so you can verify the result, and instantly re-runs the maths when you change the BAC water volume or syringe size — which is why it's the most-used research utility on the site.

Yes — the peptide calculator is completely free, requires no sign-up, and runs entirely in your browser on desktop and mobile. Nothing you enter is stored or sent anywhere. It is provided by Peptide Warehouse Australia as a research aid for in-vitro laboratory use only.

For in-vitro laboratory research only. The Peptide Reconstitution Calculator is a volumetric arithmetic aid. It does not recommend research protocols, doses, or schedules. Always verify any calculation independently against your research protocol or the published literature for the compound. Products supplied by Peptide Warehouse Australia are not for human consumption, therapeutic use, or veterinary use. Purchasers must be 18 years of age or older.